Duangjan K., Nakkhunthod W., Pumas C.

Photoautotrophic production of hydrogen in Carteria crucifera AARL G045 co-cultured with bacterial flora

Abstract This study reports on high potential of photoautotrophic biohydrogen production in co-culture of the novel hydrogen producing microalga Carteria crucifera AARL G045 with its bacterial flora. Hydrogen production from immobilised and free cells were compared under photoautotrophic and photofermentation conditions. Two bacterial floras of Carteria crucifera AARL G045 cultures were isolated and identified by 16S rDNA sequence analysis as Agrobacterium tumefaciens G045-01 and Aeromicrobium kwangyangensis G045-02. As a result, co-culture of Carteria crucifera AARL G045 and A. kwangyangensis G045-02 generated higher level of hydrogen gas than that of the mono algal culture by about 1.32 times. In addition, co-culture of both free cells of the microalga and the bacterium produced higher hydrogen than both co-immobilisation in calcium alginate by about 2.04 times.

Doi https://doi.org/10.2478/botlit-2019-0016

Keywords biohydrogen, co-cultivation, free cells, immobilised cells, microalgae

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